Cyanobacterial resources and protocols.

Cyanocyc

CyanoCyc is a web portal that integrates an exceptionally rich database collection of information about cyanobacterial genomes with an extensive suite of bioinformatics tools. It was developed to address the needs of the cyanobacterial research and biotechnology communities. The 277 annotated cyanobacterial genomes currently in CyanoCyc are supplemented with computational inferences including predicted metabolic pathways, operons, protein complexes, and orthologs; and with data imported from external databases, such as protein features and Gene Ontology (GO) terms imported from UniProt. Five of the genome databases have undergone manual curation with input from more than a dozen cyanobacteria, including from the Lea-Smith lab, with experts to correct errors and integrate information from more than 1,765 published articles. CyanoCyc has bioinformatics tools that encompass genome, metabolic pathway and regulatory informatics; omics data analysis; and comparative analyses, including visualizations of multiple genomes aligned at orthologous genes, and comparisons of metabolic networks for multiple organisms.

Relevant paper: https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1340413/full

Website: https://cyanocyc.org/

Transformation of cyanobacteria.

Click below for a detailed video on performing transformations in the model cyanobacterium Synechocystis sp. PCC 6803 and Synechococcus sp. PCC 7002.